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Figure 2 | BMC Molecular Biology

Figure 2

From: Biochemical characterization of a recombinant Japanese encephalitis virus RNA-dependent RNA polymerase

Figure 2

RdRp assay using a poly(A) template and oligo(U) 20 primer. (A) Primer-dependent RNA synthesis. RdRp assays were performed with the purified JEV NS5 using a poly(A) RNA template in the presence (+) or absence (-) of the primer oligo(U)20. (B) RdRp assays were performed with the purified wild-type NS5 (GDD) and its mutant NS5D668A (GAD) in the presence (+) or absence (-) of a poly(A) RNA template. Relative RdRp activities (%), which were obtained by comparing the 32P-UMP incorporation measured by liquid scintillation counting with that obtained for the reaction with the template and primer, 3.0 × 105 cpm, are presented.

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