Figure 9

Induction of srxn1 expression by metabolic stimuli in isolated rat islets. Rat islets were isolated, cultured, and serum deprived at reduced glucose concentration (1 mM) for 20 hours. Stimulation was done for indicated period of time with high glucose (25 mM) plus cpt-cAMP (0.2 mM) or GLP-1 (10 nM). Transcript levels of the AP-1 target gene srxn1 were quantified by real-time RT-PCR, normalized with reference to 18S rRNA, and shown as relative values. Shown are the means of values obtained for at least three experiments. Student T-tests were used for statistical analysis; *p < 0.05 vs non-stimulated; ** p < 0.01 vs non-stimulated.