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Figure 6 | BMC Molecular Biology

Figure 6

From: Transcriptional response of pancreatic beta cells to metabolic stimulation: large scale identification of immediate-early and secondary response genes

Figure 6

Srxn1 is a transcriptional target of AP-1. A) Depicted srxn1 reporter constructs were transfected in Min6 cells. Results as means of four independent experiments, with s.d. as error bars. *, p < 0.01 vs control; #, p < 0.01 vs corresponding non-stimulated condition, by Student T-test. B) Depicted srxn1 reporter constructs were co-transfected respectively with A-FOS, A-C/EBP, or a control expression vector. Results as means of four independent experiments, with s.d. as error bars. *, #, p < 0.01, respectively p < 0.05 vs control expression vector, by Student T-test. For A) and B), stimulations were performed with 0.2 mM cpt-cAMP and 10 mM glucose for 6 hours. C) srxn1-421/+39pGL3 reporter was cotransfected with c-fos expression vector (as in Figure 5). Results as means of four independent experiments, with s.d. as error bars. *, p < 0.01 vs control expression vector, by Student T-test. D) mRNA levels for srxn1, junD and egr-1 were quantified by RT-PCR in Min6 clones stably transfected with A-FOS, control expression vector and A-C/EBP respectively. Stimulations were performed with 0.2 mM cpt-cAMP and 10 mM glucose for four hours. Four different cell preparations were analyzed for each of at least three clones in each category. Results were pooled and expressed as mean of relative mRNA levels (arbitrary units) with s.d. as error bars. *, p < 0.001 vs control, by Student T-test.

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