Validation of IEGs and targets by quantitative RT-PCR. Induction of targets is inhibited in presence of CHX, while induction of IEGs is not. Min6 cells cultured at low glucose for 20 hours were stimulated for 4 hours with 10 mM glucose and 0.2 mM cpt-cAMP, in presence or absence of CHX (5 μg/ml, added 45' prior to stimulation). mRNA levels for indicated genes were assessed by quantitative real-time RT-PCR and normalized with 18S rRNA. Results are expressed as mean of fold change compared to control condition (s.d. as error bars, n = 5). *, p < 0.01; #, p < 0.05; NS: non significant vs respective control condition (i.e with or without CHX), by Student T-test.