Interfering BgLpR expression in B. germanica by RNAi. dsBgLpR or dsControl were injected in newly emerged females, and BgLpR mRNA levels were analyzed by RT-PCR/Southern blotting in the fat body and ovaries 1, 3 and 6 days later. B. germanica actin5C levels were used as a reference. At the same time intervals, effects on apolipophorin I (ApoLpI) were also studied by Western blotting. 10 μg of total proteins (fat body) or 0.2 ovary equivalents (ovary) were loaded in each lane. Blots shown are representative of six replicates.