Figure 5From: Structural and RNAi characterization of the German cockroach lipophorin receptor, and the evolutionary relationships of lipoprotein receptorsInterfering BgLpR expression in B. germanica by RNAi. dsBgLpR or dsControl were injected in newly emerged females, and BgLpR mRNA levels were analyzed by RT-PCR/Southern blotting in the fat body and ovaries 1, 3 and 6 days later. B. germanica actin5C levels were used as a reference. At the same time intervals, effects on apolipophorin I (ApoLpI) were also studied by Western blotting. 10 μg of total proteins (fat body) or 0.2 ovary equivalents (ovary) were loaded in each lane. Blots shown are representative of six replicates.Back to article page