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Figure 4 | BMC Molecular Biology

Figure 4

From: Structural and RNAi characterization of the German cockroach lipophorin receptor, and the evolutionary relationships of lipoprotein receptors

Figure 4

Effects of juvenile hormone (JH) on BgLpR expression in B. germanica. Racemic JH III (10 μg in 1 μl of acetone) was topically applied to newly emerged females of B. germanica, and fat body and ovaries were dissected 48 h later. Controls received 1 μl of acetone. BgLpR mRNA levels were studied by semiquantitative RT-PCR/Southern blotting. B. germanica actin5C levels were used as a reference. BgLpR protein expression was studied by Western blotting. Effects on apolipophorin I (ApoLpI) were also studied by Western blotting. 10 μg of total proteins (fat body) or 0.2 ovary equivalents (ovary) were loaded in each lane. Blots shown are representative of nine replicates.

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BMC Molecular Biology

ISSN: 1471-2199

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