Effects of juvenile hormone (JH) on BgLpR expression in B. germanica. Racemic JH III (10 μg in 1 μl of acetone) was topically applied to newly emerged females of B. germanica, and fat body and ovaries were dissected 48 h later. Controls received 1 μl of acetone. BgLpR mRNA levels were studied by semiquantitative RT-PCR/Southern blotting. B. germanica actin5C levels were used as a reference. BgLpR protein expression was studied by Western blotting. Effects on apolipophorin I (ApoLpI) were also studied by Western blotting. 10 μg of total proteins (fat body) or 0.2 ovary equivalents (ovary) were loaded in each lane. Blots shown are representative of nine replicates.