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Figure 5 | BMC Molecular Biology

Figure 5

From: Histone gene expression and histone mRNA 3' end structure in Caenorhabditis elegans

Figure 5

Histone gene expression is developmentally regulated and occurs mainly during early development and in the germ line. (A) glp-4(bn2) animals were grown at 16°C or 25°C. RNA was isolated from adult animals and histone mRNA levels were analysed by Northern blotting as described. 18S rRNA was detected to control for equal loading. (B) N2 and glp-4(bn2) embryos were grown at 16°C or 25°C to L1, L3 and adult (A) stages. RNA was isolated and analysed by Northern blotting, and histone mRNA levels were determined as described. Blots were stripped between probing with histone mRNAs. For the graph, histone mRNA levels were standardised with respect to 18S rRNA, and are shown as percentage of histone mRNA in L1 animals, which was defined as 100 %.

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