Figure 2
From: Histone gene expression and histone mRNA 3' end structure in Caenorhabditis elegans
The structure of histone mRNA 3' ends: poly(A) selection of histone mRNA. Northern analysis of poly(A) selected RNA. Total RNA isolated from adult N2 animals and from Hela cells was subjected to two successive rounds of poly(A) selection using oligo-dT cellulose. 2 μg of total RNA and of poly(A) selected RNA were analysed by Northern blotting and histone H2A, H2B, H3, H4, and GAPDH mRNA levels were determined as described. Blots were stripped between probings. RNA levels were measured as described and were expressed as fold enrichment, with the RNA levels in total mRNA defined as 1. The asterisk marks the position of a H3 mRNA species enriched during poly(A) selection of C. elegans RNA.