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Figure 2 | BMC Molecular Biology

Figure 2

From: Zfra affects TNF-mediated cell death by interacting with death domain protein TRADD and negatively regulates the activation of NF-κB, JNK1, p53 and WOX1 during stress response

Figure 2

Ser8 is involved in Zfra-induced apoptosis. Ser8 is a conserved phosphorylation site in Zfra [10]. Ser8 was altered to Gly (S8G). L929 cells were electroporated with EGFP-Zfra, S8G, or EGFP, followed by culturing for 48 hr and staining with crystal violet. Transiently overexpressed Zfra induced death of L929 cells (34.8 ± 8.6%; n = 8), whereas S8G mutant had a significantly reduced activity in causing cell death (17.1 ± 5.6%; n = 8; p < 0.005, Student's t test; see *). The extent of protein expression is shown.

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BMC Molecular Biology

ISSN: 1471-2199

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