Table 1 Primers for quantitative real time RT-PCR analysis
From: ABCA1, ABCG1 and SR-BI: hormonal regulation in primary rat hepatocytes and human cell lines
Gene | GeneBank accession | Forward primer (5'-3') | Reverse primer (5'-3') | Size (bp) |
|---|---|---|---|---|
ABCA1* | Â | AACAGTTTGTGGCCCTTTTG | AGTTCCAGGCTGGGGTACTT | 157 |
h ABCG1 | NM_207630 | GGTTCTTCGTCAGCTTCGAC | GTTTCCTGGCATTCAGGTGT | 317 |
r ABCG1 | NM_053502 | GAAGGTTGCCACAGCTTCTC | CATGGTCTTGGCCAGGTAGT | 339 |
h apoA-I | NM_000039 | TGGATGTGCTCAAAGACAGC | AGGCCCTCTGTCTCCTTTTC | 190 |
r apoA-I | NM_012738 | CCTGGATGAATTCCAGGAGA | TCGCTGTAGAGCCCAAACTT | 192 |
h CYP7A1 | NM_000780 | CCTCACCACACAGTCCCTTT | CCACACCTGGCTGTAATGTG | 299 |
r CYP7A1 | NM_012942 | CGCTATTCTCTGGGCATCTC | GTACCGGCAGGTCATTCAGT | 197 |
h GADPH | AF261085 | GGCCTCCAAGGAGTAAGACC | AGGGGTCTACATGGCAACTG | 147 |
m GADPH | BC023196 | ACCCAGAAGACTGTGGATGG | CACATTGGGGGTAGGAACAC | 171 |
h SR-BI | BC022087 | CTGTGGGTGAGATCATGTGG | GCCAGAAGTCAACCTTGCTC | 216 |
r SR-BI | NM_031541 | CAAGAAGCCAAGCTGTAGGG | CCCAACAGGCTCTACTCAGC | 230 |