Figure 6
From: Do anesthetics and sampling strategies affect transcription analysis of fish tissues?
Transcriptional levels (mean normalized expression MNE) of Na+-K+-ATPase α1b in A) gill and B) brain and of Hypoxia Inducible Factor 1 (HIF1) in C) gill and D) brain tissues of Atlantic salmon differentially treated during sampling. n = 6 in all groups except n = 5 for HIF1 120 min. metacaine, HIF1 120 min. isoeugenol and Na+-K+-ATPase α1b 120 min. groups. For the HIF1 120 min. isoeugenol group, n = 4. Group identity: 0 min. seawater control (0 SW), 30 min. seawater (30 SW), 30 min. isoeugenol (30 I), 120 min. seawater (120 SW), 120 min. metacaine (120 M) and 120 min. isoeugenol (120 I). An * denotes significant differences (P < 0.05) between the 0 min. control group and the other groups (Kruskal Wallis ANOVA).