C2C12 cells recover from heat shock with a characteristic profile of injury response. Temporal gene expression profiles of heat shocked C2C12 myotubes (47°C for 30 minutes). Heat shock treatment was abated at 0 hours and cells were restored to growth conditions. mRNA was isolated at 1, 2, 4, 8 and 24 hours post-heat shock. (A-D) Transcriptional activity of the indicated genes was measured using Affymetrix GeneChips and data was analyzed using GeneSpring bioinformatics software. Data points represent the mean of three independent studies (± S.D). (E-G) C2C12 cells stably expressing Foxo3a-GFP were differentiated into myotubes (E), heat shocked and recovered for 3 hours (F) or treated with 20 μM LY294002 for 3 hours (G). Cells were then viewed under fluorescent light to observe localization of the protein.