Figure 8
From: Regulation of p73 by Hck through kinase-dependent and independent mechanisms
Hck inhibits cisplatin induced apoptosis. (A) SAOS2 cells transfected with GFP, Hck or mSH3 Hck (400 ng) were treated with 50 μM cisplatin (CDDP) for 24 hours and immunostained for Hck. The percentage of apoptotic cells was scored among expressing and non-expressing cells using morphological criteria. Data represent mean ± S.D. of at least three independent experiments. (B) SAOS2 cells transfected with Hck or mSH3 Hck (400 ng) were treated with 50 μM cisplatin (CDDP) for 24 hours and whole cell lysates were subjected to western blotting with p73 (mouse monoclonal, Imgenex) and Hck antibody. Tubulin was used as loading control. (C) Panels show the expression of GFP, Hck and mSH3 Hck expressing cells.