Skip to main content
Figure 4 | BMC Molecular Biology

Figure 4

From: Regulation of p73 by Hck through kinase-dependent and independent mechanisms

Figure 4

Hck stabilizes p73α dependent on its kinase activity. (A) Hela cells transfected with GFP (50 ng) and Hck or KDHck(200 ng) in the presence of p73α or Y28F-p73α (50 ng) were subjected to western blotting for p73, Hck and GFP. GFP was used as a transfection efficiency control. (B) Hela cells transfected with p73α or p73α and Hck together were treated with cycloheximide (50 μg/ml), harvested at the indicated time periods and immunoblotted for p73 and tubulin. The amount of DNA in transfections was kept constant by the addition of control vector pcDNA3.

Back to article page