Figure 1

Hck interacts with p73α in vitro and in vivo. (A) Cos1 cells transiently expressing HA-p73α alone or with Hck were immunoprecipitated with control rabbit IgG (C) or Hck polyclonal antibody. The immunoprecipitates were resolved by SDS-PAGE and subjected to western blotting to detect p73 and Hck. (B) GST fusion proteins bound to Glutathione-Sepharose beads were incubated with lysates of Cos1 cells overexpressing p73α and p73δ. Bound proteins were subjected to western blotting to detect p73. (C) GST-SH3 Hck and GST-mSH3 Hck recombinant fusion proteins bound to Glutathione-Sepharose beads were incubated with lysates of Cos1 cells overexpressing p73α and bound proteins were subjected to western blotting with p73 antibody. (D) Lysates of differentiated HL-60 cells (DMSO 1.25%) were immunoprecipitated with control rabbit IgG or Hck antibodies and subjected to western blotting for p73 and Hck. (WCL indicates whole cell lysate).