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Figure 3 | BMC Molecular Biology

Figure 3

From: Hin-mediated DNA knotting and recombining promote replicon dysfunction and mutation

Figure 3

Hin-mediated effect on plasmid replication. (A) Plasmid DNA was isolated from strains C600 or parC ts containing the plasmids indicated. The DNA was linearized with Hind III, which cuts all the plasmids once, including pHIN. Shown is a representative ethidium bromide-stained agarose gel from an experiment performed at 42°C. Given below the gel image are the mean plasmid level values of three independent experiments with standard deviations. (B) Plasmid DNA was isolated 50 min. after IPTG induction of Hin, nicked, displayed by high-resolution gel electrophoresis and visualized by Southern blotting. Shown is an autoradiogram. All lanes contain plasmid DNA from the pHIN-harboring parC ts cells with pBR, pREC or pKNOT. The positions of nicked dimer (), linear dimer () catenanes (e.g., ) are indicated.

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