Figure 3

Inhibition of protein synthesis selectively stabilizes ABCC5_SV1 mRNA. Total RNA was isolated from untreated ARPE-19 and Y79 cells, from cells exposed to puromycin and from cells grown in regular medium after antibiotic exposure (post-puromycin). The RNAs were subjected to qRT-PCR using primer pairs specific for the ABCC5 splice variants. The expression values for each variant are depicted by different symbols and are given relative to the expression in untreated cells which was set to 100%. Error bars denote standard deviations of reactions performed independently in triplicate.