Expression analysis of ABCC5 splice variants. (A) Virtual Northern blot probed with an RT-PCR fragment originated from exon 5B. Consequently, the 2 kb signal obtained in retina represents transcripts ABCC5_SV1, ABCC5_SV2 and ABCC5_SV3. (B) qRT-PCR expression analysis of full length ABCC5 and the three splice variants. The expression values for each transcript in the various tissues are indicated by bars in different shades and are given relative to the expression in retina which was set to 100%.