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Figure 4 | BMC Molecular Biology

Figure 4

From: Leptin induces inflammation-related genes in RINm5F insulinoma cells

Figure 4

Signalling capacity of RINm5F cells expressing LEPRb point mutants. RINm5F cells stably expressing the wild type LEPRb (YYY) or the indicated LEPRb tyrosine/phenylalanine mutants were treated with 100 ng/ml leptin (L) or vehicle (∅) for 15 min before total cell lysates were prepared. Leptin-induced phosphorylation of downstream signalling molecules was assayed by Western blotting and immunodetection with phospho-specific antibodies.

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