Effects of leptin and IL-1β on inflammation-related transcripts in RINm5F insulinoma cells. RINm5F cells stably expressing LEPRb were treated with leptin (100 ng/ml), IL-1β (50 u/ml), or a combination of both for the times indicated (1, 3, 7 or 16 h). Total RNA was isolated and subjected to Northern blot analysis with cDNA probes identified by differential hybridisation of the cDNA array (upper 6 panels, see table 1). iNOS and RPL4 serve as controls for an IL-1β-regulated mRNA and an unregulated mRNA, respectively. Gene symbols are shown in italics. A sample of degraded RNA on one of the blots is marked with an x.