Full-length NS2 expression by constitutive BSR cells and its intracellular distribution. (A) Radiolabeled lysate of uninduced (left lane) and induced WT-NS2 cell line (right lane) were immunoprecipited using polyclonal anti-NS2 antibody. The names of the cell lines are given at the top of the panel. The position of NS2 fragment is indicated. (B) Cells expressing NS2 in absence of doxycycline (left) and unmodified BSR cells (right) infected with BTV-10 at 24 h p.i were immunolabeled with an anti-NS2 polyclonal antibody (green). Nuclei were stained with Hoechst 33342.