EMSAs using nuclear extracts and 188 bp probes from acsp30 upstream sequences. Nuclear proteins were extracted from body tissue of 5-day old adult female of S strain (A) and R strain (B) and incubated with S188 (lane 2) and R188 (lane 3) probes. Free probes were run in lane 1 (S188) and lane 4 (R188). Arrows indicate the migration of complex A (slow migrating) and complex B (fast migrating). The intensities of the signals were quantified with respect to Phospholmager signals by the Image Analysis Software, ImageQuant TL (Amersham Biosciences) and represented as the percentage ratio of S188 to R188 signal intensities.