Figure 7

Assessment of promoter activity of acsp30 from R and S strains of A. culicifacies using luciferase-based reporter assay in Drosophila S2 cell line. Constructs, pGL3-Ref (702 bp and 333 bp) and pGL3-Sus (702 bp and 333 bp) were transfected in the Drosophila Schneider (S2) cell line using lipofectin and the luciferase activity measured. Promoterless vector pGL3-Basic and pGL3-Control plasmid with the SV40 promoter served as controls. Reported activities were based on three independent transfections and the data was recorded in relative light units (RLU).