Computer-assisted analysis of acsp30 upstream sequences from refractory and susceptible strains of A. culicifacies. (A) Alignment of the two sequences. The translational start site (ATG) is underlined and the numbers to the right denote the positions of the adjacent nucleotide in each line, relative to ATG at +1. The arrows indicate the boundary of the 333 bp and 702 bp constructs used for promoter analysis. The sequence marked with a dashed line represents the region of maximum dissimilarity between R and S strains.