EMSAs using nuclear extracts and 100 bp probes from acsp30 upstream sequences. Nuclear proteins were extracted from body tissue of 5-day old adult female of S strain (A) and R strain (B) and were incubated with S100 (lane 2) and R100 (lane 3) probes. Free probes were run in lane 1 (S100) and lane 4 (R100). Arrows indicate the migration of complex A (slow migrating) and complex B (fast migrating). The intensities of the signals were quantified with respect to Phospholmager signals by the Image Analysis Software, ImageQuant TL (Amersham Biosciences) and represented as the percentage ratio of S100 to R100 signal intensities.