(A) Deduced amino acid sequence of ACSP30 from A. culicifacies. The 816 bp cDNA encoded a 272 amino acid protein. The three residues of the catalytic triad are marked by 'Δ'. The putative 17 amino acid signal peptide is underlined with a solid line and the putative 19 amino acid propeptide is underlined with a dotted line. '' indicates the cleavage site of signal peptide and '▼' shows the activation site. The conserved cysteines are shown in bold and larger font. (B) Phylogenetic tree describing the sequence similarity of insect serine proteases. The tree was constructed by the neighbour-joining method with the Best Tree mode. A value of 0.1 corresponds to a difference of 10% between two sequences. GenBank™ accession numbers are as follows; A. gambiae predicted serine protease (agCP8348), AAAB01008980.1; A. gambiae serine protease (AgSp24D), U21917: A. gambiae immune-responsive chymotrypsin-like serine protease-related protein (ISPR1), AF203336: A. gambiae EASTER-like serine protease (Sp14D1), AF007166: Culex quinquefasciatus serine protease (CULQU), Q23731: Chrysomya bezziana serine protease K17/F1R1 (CHRBE), Q9GSM5; Drosophila yakuba serine protease (DROYA), Q6X139.