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Table 2 Oligonucleotide primers designed for RACE-PCR and for the selection of positive clones

From: Selection strategy and the design of hybrid oligonucleotide primers for RACE-PCR: cloning a family of toxin-like sequences from Agelena orientalis

Primer name

Primer sequence

URA_L*

CTGCAGATCTAGAAGCTTTTTTTTTTTTTT

URA_N

GGAATTCTGCAGATCTAGAAG

URA_T

GGAATTCTGCAGATCTA

OLIGO-dT-21**

TTT TTT TTT TTT TTT TTT TTT

OLIGO-dT-24

TTT TTT TTT TTT TTT TTT TTT TTT

OLIGO-dT-27

TTT TTT TTT TTT TTT TTT TTT TTT TTT

hGAP-F62***

AGCCAAAAGGGTCATCATCT

hGAP-R62

GCCTGCTTCACCACCTTC

pBS-F****

GGTTTTCCCAGTCACGA

pGM-2R

ATGCTTCCGGCTCGTATGT

  1. * URA (all primers) – RACE-PCR primers;
  2. ** OLIGO-dT (all primers) are for RACE-PCR, OLIGO-dT-21 was also used for cDNAs synthesis;
  3. *** hGAP (both primers) – for positive controls (with mouse cDNA);
  4. **** pBS-F and pGM-2R designed for the selection of positive clones from bacterial colonies.