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Figure 2 | BMC Molecular Biology

Figure 2

From: Selection strategy and the design of hybrid oligonucleotide primers for RACE-PCR: cloning a family of toxin-like sequences from Agelena orientalis

Figure 2

Multialignment of the protein toxin sequences obtained form Genbank and SwissProt. Over 150 toxin and toxin-like sequences were available in the beginning of investigation and were aligned to identify potential conservative sequence motifs. 51 sequences shown aligned (top panel). The five sequences (TX2I, TX2II, TX2III, TX3-4-61 and NEUROTOX from Phoneutria nigriventer (Brazilian armed spider)) selected to exemplify a conservative motif "T/DCDCCG". Bottom panel exemplifies the strategy of primer design.

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