The distribution of active histone modifications on differently active gene promoters in asynchronized (A) and mitotic (M) MEL cells. A. H3 and H4 acetylation. B. H3-K4 and H3-K79 dimethylation Comparative. ChIP analyses were performed with the equivalent DNA input amounts of chromatin from asynchronous and mitotic cells, then analyzed by PCR (In-A and In-M). PCR primer pairs flank the promoter regions of transcriptionally active, competent and inactive genes. The relative level of histone modifications at promoters of the individual genes in A and M cell populations were analyzed after normalizing the DNA amount differences in A and M cell populations through DNA input (In-A and In-M).