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Figure 6 | BMC Molecular Biology

Figure 6

From: Antisense oligonucleotide-induced alternative splicing of the APOB mRNA generates a novel isoform of APOB

Figure 6

Transfection of APOB skip 27 ASOs causes expression of a short isoform of APOB, APOB87 SKIP27 . HepG2 cells were transfected with the indicated ASOs at 250 nM, then pulse-labelled with Trans-35S-Label for 1 hour in the presence of oleic acid-BSA, which stimulates APOB100 expression [62]. Cell lysates or cell culture media were immunoprecipitated with a polyclonal antibody for APOB. The immunoprecipitates were subjected to SDS-PAGE (4% Tris-glycine), followed by autoradiography. The gel shown is representative of three experiments. A short isoform of APOB, APOB87SKIP27, is seen with the skip 27-3B ASO. This short isoform is not seen with the 3B scram ASO, nor with the no-oligo control. This matches the presence and absence of skip 27 mRNA seen with these ASOs.

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