Skip to main content

Table 1 Sequences of primers used for the constructs and the LightCycler

From: Deletion of the cruciform binding domain in CBP/14-3-3 displays reduced origin binding and initiation of DNA replication in budding yeast

Primers

Sequences

α5-helix deletion

 

Fragment 1-F

5' CGGAATTCATGGATGATCCGAGAGGATCTG 3'

Fragment 2-F

5' GCTCTAGATCACTGATTTTCGTCTTCCAC 3'

Fragment 1-R

5' CGCCTTGGACTCGCCAGTGTTAGCTGC 3'

Fragment 2-R

5' GCCCAAGGCCACAGGAAACGACAGGAAGG 3'

PCR of ARS307

 

ARS307-F

5' ATATTGCAATTACTTCTTCTCATGCAC 3'

ARS307-R

5' ATATTGCAATTACTTCTTCTCATGCAC 3'

PARS307-F

5' GGTAGGGATAATAATCTGTAATGAGGA 3'

PARS307-R

5' TATGTCGACGAGTGAGTTGGA 3'

Real-Time PCR

 

RT-ARS307-F

5' GCAGTAGCACATGGACACATTCAC 3'

RT-ARS307-R

5' AGGGTTTCTGGAGAGGTAGGGATA 3'

NegARS307-F

5' ATTTTGTGGTGGATTGGAG 3'

NegARS307-R

5' GCTTGTTTGTGCAACTAAACG 3'

ARS1-F

5' GAAAATAGGTTATTACTGAGTAG 3'

ARS1-R

5' CCTGCGATGTATATTTTCCTG 3'

R2.5-F

5' CATCAATTGTGCACTCGGAC 3'

R2.5-R

5' GAACACGGCAATTGTAGGTGG 3'