Western blot analysis of wild-type and mutant 14-3-3ε proteins. A- Whole cell extracts (WCE) of the wild-type(14-3-3ε), the α5-helix-deleted (14-3-3-α5) 14-3-3ε, as well as Bmh1/Bmh2 and GF14 proteins-expressing cells, were prepared and 20 μg of total protein of each were subjected to electrophoresis on a 12% SDS polyacrylamide gel. All proteins were revealed by probing with anti-14-3-3ε antibody and anti-Bmh1 antibody. B- Expression of the wild type and the mutant 14-3-3ε in the presence and absence of formaldehyde, detected by anti-14-3-3ε antibody. C- Quantification of the immunoblots after normalization for the formaldehyde crosslinked 14-3-3ε.