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Table 2 Relative expression of Giardia intestinalis core histone genes

From: Core histone genes of Giardia intestinalis: genomic organization, promoter structure, and expression

Gene Accession #1 PCR primers (5' → 3')2 Avg. PCR efficiency3 Avg. Ct4
H4 AF139876 F: AGACCCGCGAGGTCCTCAA 91 ± 9% 15.9 ± 0.8
   R: TTGCGCTGGCCGTGTTCTG   
H3 AF139875 F: TACCAGAAGTCCACAGACC 95 ± 10% 16.0 ± 0.6
   R: TGGAAGCGGATGTCGGA   
H2a AF139873 F: GTCGTGGCAGAGGTCTT 102 ± 15% 16.9 ± 1.2
   R: CTCCTTGTCCTTGCGGA   
H2b AF139874 F: GACAACATCCGCTCCGA 101 ± 16% 17.8 ± 1.8
   R: CGAAGAGGTCGTTCACG   
  1. 1 Accession number for nucleotide sequence in GenBank
  2. 2 The sequences are denoted F for the forward primer, and R for the reverse primer.
  3. 3 Average PCR amplification efficiencies and standard deviations are calculated from triplicate reactions performed in 3 to 5 independent experiments.
  4. 4 Average Ct values and standard deviations are calculated from triplicate reactions performed in 3 to 7 independent experiments.