Core histone genes of Giardia intestinalis: genomic organization, promoter structure, and expression

BMC Molecular Biology

Table 2 Relative expression of Giardia intestinalis core histone genes

Gene	Accession #¹	PCR primers (5' → 3')²	Avg. PCR efficiency³	Avg. C_t⁴
H4	AF139876	F: AGACCCGCGAGGTCCTCAA	91 ± 9%	15.9 ± 0.8
		R: TTGCGCTGGCCGTGTTCTG
H3	AF139875	F: TACCAGAAGTCCACAGACC	95 ± 10%	16.0 ± 0.6
		R: TGGAAGCGGATGTCGGA
H2a	AF139873	F: GTCGTGGCAGAGGTCTT	102 ± 15%	16.9 ± 1.2
		R: CTCCTTGTCCTTGCGGA
H2b	AF139874	F: GACAACATCCGCTCCGA	101 ± 16%	17.8 ± 1.8
		R: CGAAGAGGTCGTTCACG

¹ Accession number for nucleotide sequence in GenBank
² The sequences are denoted F for the forward primer, and R for the reverse primer.
³ Average PCR amplification efficiencies and standard deviations are calculated from triplicate reactions performed in 3 to 5 independent experiments.
⁴ Average C_t values and standard deviations are calculated from triplicate reactions performed in 3 to 7 independent experiments.

ISSN: 1471-2199