Figure 3
From: Core histone genes of Giardia intestinalis: genomic organization, promoter structure, and expression
Characterization of protein binding to the him sequence. A,Gel-shift assays were performed with 5' biotin-labeled, double-stranded DNA containing three histone motifs in tandem (3him) and crude nuclear extract from Giardia. Lane 1 contains the probe alone; lane 2 contains the probe plus nuclear proteins; lanes 3–11 contain unlabeled DNA used at 100X molar excess relative to the probe. B, The probe and competitors were generated by annealing complementary oligonucleotides together, although only the sequences of the plus strands are shown. DNA motifs within the sequences are indicated: grey box for him, open box for the AT-rich element; g-CAB elements are underlined; and the TATA-box of the E1B adenovirus is overlined.