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Figure 2 | BMC Molecular Biology

Figure 2

From: Optimization of laser capture microdissection and RNA amplification for gene expression profiling of prostate cancer

Figure 2

Yield and integrity of RNA isolated from laser capture microdissected cells. (Left axis), 3'/M beta-actin ratios determined by qPCR as a measure of the quality of RNA samples obtained by LCM of a total of 27 cases of benign prostate, benign prostatic hyperplasia (BPH), primary Gleason pattern 3 (GP3), pattern 4 (GP4), pattern 5 (GP5), and metastatic prostate cancer (Met). (Right axis), relative yields of LCM RNA samples based on qPCR with primers specific for the 3' end of the beta-actin transcript.

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