Table 1 Primer sequences used in this study.
Name | Sequence (5'→3') a | Experiment b |
|---|---|---|
hPP rev (+207/+178) | CACATCGTTCCCGTTGAGTTGTTGCTCTCC | RT-PCR |
hPP 1 (+1/+19) | AACTGCAAAGTTTGCTGTC | RT-PCR |
hPP 2 (-16/+3) | CGCTCGGAAAGTTCTCAAC | RT-PCR |
hPP 3 (-35/-17) | GAGAGATAAATGCTGACTC | RT-PCR |
hPP 4 (-52/-33) | CTCCCCCGCCTCCTCGGGA | RT-PCR |
hPP 5 (-68/-50) | CTTGCGGCCGACCCCGCTC | RT-PCR |
hPP 6 (-83/-65) | CCGGACGGAGACCACCTTG | RT-PCR |
hPP 7 (-97/-79) | CGGGCCTAGCGCGCCCGGA | RT-PCR |
hPP 8 (-108/-90) | GAGCCCGAGGGCGGGCCTA | RT-PCR |
hPP 9 (-125/-107) | TTGGCGCCGGCCAAACAGA | RT-PCR |
hPP rev (+114/+96) | AGCCTGGAGGAGCGCGAC | RT-PCR |
FII (-1885/-1866) | GCAATGAGCTCATCCACTAACTCCTTCATCC | Cloning, LA |
F I (-857/-840) | GCAATGAGCTCACGTAAGTTCTCTGGTTG | Cloning, LA |
RII (-783/-803) | GCAATCTCGAGAATCCTCACTTTACTGGTTTA | Cloning |
RI (+154/+171) | GCAATCTCGAGCCGAGCAGCAAGATTCTG | Cloning, LA |
F1 (-1642/-1616) | TTGAATAAAGCGGGCCATC | LA |
F2 (-1378/-1360) | TCATCTTCTAGGTTCAGGT | LA |
F3 (-1104/-1086) | ACAATCTCTCCTGTGATTC | LA |
F4 (-666/-658) | ACAGCATCGCAGCGCTGGA | LA |
F5 (-498/480) | ACCAGCGCGGGCCGCCTCT | LA |
F6 (-361/-343) | CACTTCTGCCCATAACAAG | LA |
F7 (-261/-243) | AACCCCGTTTGTTGCATGT | LA |
F8 (-232/-214) | CCTCCTGTTGTTGAGCAGA | LA |
F9 (-186/-168) | AGTCCTGGCGGCCCCCGCA | LA |
F10 (-159/-141) | CCTGTAACTTTAAACCTGG | LA |
F11 (-114/-96) | CAAACAGAGCCCGAGGGCG | LA |
F12 (-76/-58) | GAGACCACCTTGCGGCCGA | LA |
F13 (-38/-20) | CGGGAGAGATAAATGCTGA | LA |
F14 (-26/-8) | ATGCTGACTCCGCTCGGAA | LA |
fp 1 | CCGCTCCCCCGCCTCCT CGGGA | EMSA |
fp 1 mut | CCGCTCCA CATAA TCCTCGGGA | Mutation |
fp 2 | GAGCCCGAGGGCGGG CCTAGCG | EMSA |
fp 2 mut | GAGCCCGAT GATAA GCCTAGCG | Mutation |
fp 3 | GATCATCGTTTTGGCGCCGGC CAA | EMSA |
fp 4 | CAGATGACGGCCAACTTTTTTTGTTTTTAAGCCATCAAA | EMSA |
fp 5 | CCAAACTCCCCCCACC AGCCAG | EMSA |
fp 5 mut | CCAAACTCTA CAG ACCAGCCAG | Mutation |
fp 6 | TTTTCAAAACTGCCAAAGCC | EMSA |
fp 7 | CACCGCTTGGCCCGGCCTAATCC | EMSA |
fp 7 mut | CACCGCTTGGAT CTTG CCTAATCC | Mutation |
fp 8 | GGACACAGGTTTGCGACCAGGACGGT | EMSA |
Bis-fwd 1 | GTTAGGGTTTGTAGTTTTGGTGG | BS |
Bis-rev 1 | CACCACAACCACAAACCCAACCT | BS |
Bis-fwd 2 | GAGGGTGAATTAGGTTTGGAAGGGG | BS |
Bis-rev 2 | CAACCCACACTAATCAATTACACCC | BS |
Bis-fwd 3 | GAGTTGTTGGTTTTATTTGGTTTAGGAGGGTGAG | BS |
Bis-rev 3 | CTATTCTACTTATATATAACTCCAAAATCACACAC | BS |