Figure 10

Possible model of transcriptional control of podoplanin expression in MG63 versus Saos-2 cells. The PDPN promoter is active, when, accompanied by distal hypermethylation, several factors accumulate near Sp1/Sp3 proteins bound at sites bp -700/-696 (Sp1.4) and bp -100/-95 (Sp1.2). A factor located downstream from bp -38 and associated with Sp1/Sp3 bound to site Sp.1 may exert a repressive effect. The arrow indicates the major transcription start site; the small vertical bars indicate the CpG sites; the diamonds indicate the CpG sites analyzed in this study; fp, footprint detected protein; MDF, methylation dependent factor.