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Figure 3 | BMC Molecular Biology

Figure 3

From: Two non-homologous brain diseases-related genes, SERPINI1 and PDCD10, are tightly linked by an asymmetric bidirectional promoter in an evolutionarily conserved manner

Figure 3

Analysis of the intergenic distance between PDCD10 and SERPINI1 and its promoter activity in various cell lines. A) The intergenic distance between PDCD10 and SERPINI1 was 851 bp, as determined from the results of 5'-RACE. The nucleotides between the two closest transcriptional start sites of PDCD10 and SERPINI1 are numbered from the centromeric (cen) end to the telomeric (tel) end. B) The promoter activity of the intergenic 851-bp fragment in either direction (toward PDCD10 or SERPINI1) was determined by the firefly luciferase assay in 6 different cell lines including human neuroglioma H4, human brain glioblastoma U-87 MG, human ovarian adenocarcinoma OVTW-59-4, human lung adenocarcinoma CL1-5, human cervical adenocarcinoma HeLa, and rat adrenal gland pheochromocytoma PC-12 cells.

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BMC Molecular Biology

ISSN: 1471-2199

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