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Figure 2 | BMC Molecular Biology

Figure 2

From: Two non-homologous brain diseases-related genes, SERPINI1 and PDCD10, are tightly linked by an asymmetric bidirectional promoter in an evolutionarily conserved manner

Figure 2

Relative expression levels of SERPINI1 and PDCD10 between normal and tumorous tissues. A) Semi-quantitative RT-PCR analysis of SERPINI1 and PDCD10 transcripts. RNAs used in this study were obtained from the normal human brain (Lanes 2, 6 & 10), human neuroglioma H4 cells (Lanes 3, 7 & 11) and human glioblastoma U-87 MG cells (Lanes 4, 8 & 12). M indicates the DNA size markers; and Lanes 1, 5 & 9 are the negative controls without templates in the PCR reactions. B & C) Northern hybridization analysis of PDC D10 (B) and ubiquitin (C) in paired normal/tumor tissue samples (BD Clontech). The array order of all tissues examined is shown in panel D.

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