Figure 2From: Two non-homologous brain diseases-related genes, SERPINI1 and PDCD10, are tightly linked by an asymmetric bidirectional promoter in an evolutionarily conserved mannerRelative expression levels of SERPINI1 and PDCD10 between normal and tumorous tissues. A) Semi-quantitative RT-PCR analysis of SERPINI1 and PDCD10 transcripts. RNAs used in this study were obtained from the normal human brain (Lanes 2, 6 & 10), human neuroglioma H4 cells (Lanes 3, 7 & 11) and human glioblastoma U-87 MG cells (Lanes 4, 8 & 12). M indicates the DNA size markers; and Lanes 1, 5 & 9 are the negative controls without templates in the PCR reactions. B & C) Northern hybridization analysis of PDC D10 (B) and ubiquitin (C) in paired normal/tumor tissue samples (BD Clontech). The array order of all tissues examined is shown in panel D.Back to article page