Figure 6
From: NF-κB mediates the transcription of mouse calsarcin-1 gene, but not calsarcin-2, in C2C12 cells
The activation of CS-1 and CS-2 promoters by different signalling molecules. A. Deleted promoter constructs of CS-1 and CS-2 were used in the NF-κB p50 cotransfection analysis. A schematic representation of these two promoters is shown. These vectors were cotransfected with pReceiver-NF-κB1. B. A range of different truncated promoter constructs (pCS1-2554/-144, pCS1-926/-144 and pCS2-2478/-67) were cotransfected with pcDNA-NFATc4. A consensus NFAT element in the region -1206 to -926 was removed in the pCS1-926/-144 vector. C. The two wild type calsarcin promoters were cotransfected with pReceiver-MEF2C. The pNF-κB-Luc, pNFAT-Luc and pMEF2-Luc vectors were used as controls. Values are expressed as ratios of the basal activity (resulting from cotransfection with an empty pcDNA3.1 vector) and represent the mean ± SD for two sets of independent experiments, each performed in triplicate. (Note: comparison of luciferase activity between different vectors is not valid.)