Expression of bovine RTEL. Expression of RTEL in blastocyst. Lines 1 to 4 indicates: marker, GAPDH amplicon, RTEL first round PCR and RTEL second round PCR. GAPDH serves as standard control, arrows highlight the amplification signals. B, C. RTEL expression in indicated adult tissues and cultured cells. A Two kb amplicon of bovine RTEL cDNA and 600 bp of GAPDH were labeled using [32P]dCTP to hybrid to normalized total RNA from multiple tissues and cells. The above panel shows ~4.5 kb hybridization signals of indicated tissues and cells, lower panel shows the ~1.3 kb GAPDH Northern blot signals as standard control.