Figure 1

Genomic organization, motif characterization and sequence comparison of bovine RTEL. Bovine RTEL encodes a conserved helicase-like protein. A. Schematic representation of genomic organization and motif characterization of bovine RTEL. The genomic sequence of bovine RTEL is distributed among 35 exons spanning 24.8 kb of genomic DNA. The vertical lines represent exons (only partial exons are numbered); grey lines indicate CpG islands predicted in the putative promoter region. Because two forms of 5'UTR were identified, the exon 1' and exon 2' represent the alternative first and second exons respectively (see alternative splicing section for details). The deduced bovine RTEL protein contains three functional domains (DEXDc2, DEAE_2 and HELICc2) classified into the DinG, Rad3-related DNA helicases. The grey lines and related numbers below the schematic (not to scale) represent the position of each domain. The roman numbers specify seven characterized helicase motifs within the domains, and "PIP" indicates the PIP box identified near the C-terminus of RTEL protein. B. multiple alignments of deduced amino acids sequences in the eight identified motifs among three identified RTEL homologues. The consensus sequences of each motif are shown, identical and similar residues are shaded by black and grey respectively.