Figure 2
From: A cryptic promoter in potato virus X vector interrupted plasmid construction
The cryptic promoter in the PVX vector. The transcription of the foreign gene gp53 was detected with Northern blots. Lane 1: 100 ng total RNA from the pgR106gp53 transformed E. coli. Lane 2: 100 ng total RNA of the pgR106 transformed E. coli (without foreign gene). The specific gp53 band was detected. Lane 3: 300 ng RNA from the pgR106gp53 transformed E. coli. Lane 4: 300 ng total RNA from the pgR106 transformed E. coli.