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Figure 1 | BMC Molecular Biology

Figure 1

From: Distinctive expression patterns of 185/333 genes in the purple sea urchin, Strongylocentrotus purpuratus: an unexpectedly diverse family of transcripts in response to LPS, β-1,3-glucan, and dsRNA

Figure 1

Expression of 185/333 in response to various antigens. Nine animals were injected with either (A) LPS (n = 3), (B) Lam (n = 3), (C) dsRNA (n = 3), or (D) aCF (n = 2). Whole coelomic fluid (wCF) samples were collected prior to injection (pre) and at various times after challenge. The expression of both 185/333 (upper gels) and Sp056 (lower gels) were analyzed by RT-PCR in separate reactions and the agarose gels are shown. The vertical arrows indicate the time points at which the animals received injection(s). All animals received at least one injection at 0 h. Based on immune activation responses, three animals received second injections; animal 2-LPS and animal 5 at 24 h, animal 6 at 72 h.

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