Figure 6

Functional roles of DmSnR60 molecules. (A) Base-pairing interactions between DmSnR60 and Drosophila 28S rRNA sequences. The upper strand represents the rRNA sequence; the G1083 and the A1092 residues selected for methylation by D' and D antisense elements are marked by the asterisk. (B) Reverse transcription at low dNTP concentration of the 28S Drosophila rRNA region, including the G1083 and the A1092 methylation sites; on the left, lanes T, C, G and A are dideoxy sequence reactions performed using the primer utilised for reverse transcription and a plasmid carrying the Drosophila 28S rRNA gene. One additional 2'-O-methylation, at position Gm1108 of 28S rRNA, is detected in this experiment. This methylation has not yet been described in other organisms and may be specific to Drosophila rRNA.