Figure 6
Effects of Ets-2 and activated PKA on the Tkdp-1 1000 bp promoter activity in JEG-3 cells. Expression of the 1000 bp promoter-Luc construct A) following co-transfection with activated PKA, activated PKA+PKI, mutPKA, Ets-2, Ets-2+activated PKA, Ets-2+activated PKA+PKI, Ets-2+mutPKA and B) in cells exposed to the PKA inhibitor H89 in presence and absence of co-transfected Ets-2. Luc activities are expressed relative to that of the basal activity of the 1000 bp construct and data are shown as mean ± SEM (n = 3). The following values are significantly different at P < 0.05: A) 1000 vs. 1000+PKA, 1000+Ets-2, 1000+Ets-2+PKA; 1000+PKA vs. 1000+mutPKA, 1000+PKA+PKI; 1000+Ets-2+PKA vs. 1000+Ets-2+mutPKA, 1000+Ets-2+PKA+PKI and B) 1000+PKA vs. 1000+PKA+3 μMH89, 1000+PKA+8 μMH89; 1000+PKA+12 μM H89; 1000+Ets-2+PKA vs. 1000+Ets-2+PKA+3 μM H89, 1000+Ets-2+PKA+8 μM H89, 1000+Ets-2+PKA+12 μM H89