Figure 4

Transactivation of the Tkdp-1 promoter by Ets-2. A) Effects of different Ets family transcription factors on reporter expression from the 1000 bp Tkdp-1 promoter-Luc reporter construct in JEG-3 cells. Luc activities (means ± SEM; n = 3) are expressed relative to that of the basal activity of the 1000 bp construct. Following values differ significantly (p < 0.05): 1000 vs. 1000+Ets-1 and Ets-2; 1000+Ets-1 vs. 1000+Ets-2. B) Effect of Ets-2 on the transcriptional activities of three 5^/-truncated Tkdp-1 promoter-Luc reporter constructs 1000, 352 and 140 in JEG-3 cells. Luc activities are expressed relative to that of the basal activity of each of the truncated constructs and data are shown as mean ± SEM (n = 3). For each truncated construct, the fold-induction in reporter activity after co-transfection with Ets-2 is significantly different from that without Ets-2 (P < 0.05). C) Effect of increasing concentrations of Ets-2 on the activity of the 1000 bp Tkdp-1 promoter-Luc reporter construct. Luc activities are expressed relative to that of the basal activity of the 1000 bp construct and data are shown as mean ± SEM (n = 3). Following values differ significantly (p < 0.05): 1000 vs. 0.5 μg, 0.9 μg, 1.3 μg and 1.5 μg Ets-2.