Figure 10
EMSA with 32P-labeled C/EBP oligonucleotide (-117 to -109) and JEG-3 cell extracts (CE). Lane 1: labeled oligonucleotide+CE; lane 2: labeled oligonucleotide-CE; lane 3: labeled oligonucleotide+CE+C/EBP-α IgG; lane 4: labeled oligonucleotide+CE+C/EBP-β IgG; lane 5: labeled oligonucleotide+CE+C/EBP-δ IgG; lane 6: labeled oligonucleotide+CE+non-immune rabbit IgG; lanes 7–8: labeled oligonucleotide+CE+250-fold molar excess of unlabeled wild type (lane 7) and mutated (lane 8) C/EBP competitor oligonucleotides.