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Table 2 Experimental design

From: Selection of reliable reference genes for qPCR studies on chondroprotective action

Experimental condition

Supplements on days of culture

 

day 5

day 6

Control

-

-

IL-1β

-

IL-1β

Simultaneous incubation with CPA [c]1

-

[c]1+IL-1β

Simultaneous incubation with CPA [c]2

-

[c]2+IL-1β

Pre-incubation with CPA [c]1

[c]1

[c]1+IL-1β

Pre-incubation with CPA [c]2

[c]2

[c]2+IL-1β

CPA only

[c]2

[c]2

  1. Supplements added to the chondrocyte culture medium on days 5 and 6 of the culture period are presented. Either glucosamine, curcumin or diacerein were used as chondroprotective agent (CPA) in 3 separate experiments. Each experiment followed the presented setup of 7 different study groups.
  2. IL-1β, 10 ng/ml recombinant human interleukin-1beta; [c]1, low concentration of glucosamine (50 μM), curcumin (5 μM) or diacerein (5 μM); [c]2, high concentration of glucosamine (5 mM), curcumin (50 μM) or diacerein (50 μM).
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BMC Molecular Biology

ISSN: 1471-2199

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