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Figure 6 | BMC Molecular Biology

Figure 6

From: Interleukin-4 inhibition of interleukin-1-induced expression of matrix metalloproteinase-3 (MMP-3) is independent of lipoxygenase and PPARγ activation in human gingival fibroblasts

Figure 6

PPARγ ligand 15dPGJ2 also induces MMP-3 transcription in COS-1 cells. A) HGF cultures were pre-treated with 15dPGJ2 at the indicated doses for one hour before addition of IL-1 (10 ng/ml) and/or IL-4 (10 ng/ml). Total RNA was isolated at 12 hours, quantitated by real-time PCR and normalized to levels of GAPDH. The graph represents data from six independent experiments performed in triplicate, mean +/- SEM. B) COS-1 cells were transiently transfected with a luciferase reporter construct containing 2300 bp of the human MMP-3 5' flanking sequence, along with an SV-β-gal reporter as a control for transfection efficiency. 15dPGJ2 was added 16 hours after transfection, and cells were harvested 24 hours later. Shown are data from three independent experiments performed in triplicate, normalized to levels of β-galactosidase, and expressed relative to control, mean +/- SEM. Statistical significance was determined using Student's t-test.

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